NAME: SOLpro FUNCTION: predict protein solubility upon overexpression NOTE: assume that the path of PPVED is '/datadisk/PPVED-1.0' ================================================================ #step1: Change the current working directory to the path of PPVED cd /datadisk/PPVED-1.0 #step2: Download and decompress SOLpro cd software wget -c http://download.igb.uci.edu/solpro.tar.gz tar zxvf solpro.tar.gz #step3: Install SOLpro install SSpro 4.0 #when you installed DIpro 2.0, you had already completed the installation of SSpro 4.0 #therefore, here, you can skip the installation of SSpro 4.0 install DOMpro 1.0 wget -c http://download.igb.uci.edu/dompro1.0.tar.gz tar zxvf dompro1.0.tar.gz cd dompro1.0 open script 'configure.pl', and set the DOMpro path ($install_dir, line 17) and SSpro4 path ($pspro_dir, line 20) to their installation directories e.g. : $install_dir = "/datadisk/PPVED-1.0/software/dompro1.0/"; e.g. : $pspro_dir = "/datadisk/PPVED-1.0/software/sspro4/"; ./configure.pl install LibSVM cd .. wget -c https://www.csie.ntu.edu.tw/~cjlin/libsvm/libsvm-3.24.zip #Alternatively, you can also download from our site: wget -c http://www.ppved.org.cn/dependent/other_programs/libsvm-3.24.zip unzip libsvm-3.24.zip cd libsvm-3.24 make install SOLpro cd ../solpro open script 'configure.pl', and set the SSpro4 path ($sspro_dir, line 21), DOMpro path ($dompro_dir, line 24) and SOLpro path ($solpro_dir, line 27) to their installation directories e.g. : $sspro_dir = "/datadisk/PPVED-1.0/software/sspro4/"; e.g. : $dompro_dir = "/datadisk/PPVED-1.0/software/dompro1.0/"; e.g. : $solpro_dir = "/datadisk/PPVED-1.0/software/solpro"; cp ../libsvm-3.24/svm-scale bin cp ../libsvm-3.24/svm-predict bin cd scripts open script 'features1.c', and comment out its line 68, that is, add character // before line 68 open script 'features2.c', and comment out its line 47, that is, add character // before line 47 cd .. ./configure.pl #step4: test SOLpro cd test cp ../../../library/lib*.so.? . cp ../../testdata/test.fa . cp ../../testdata/test.solpro . ../bin/predict_sol.sh test.fa test.output #results: the output file 'test.output' should be identical to the provided file 'test.solpro'. Congratulations, the installation is complete !