NAME: UbPred FUNCTION: predict protein ubiquitination sites NOTE: assume that the path of PPVED is '/datadisk/PPVED-1.0' ================================================================ #step1: Change the current working directory to the path of PPVED cd /datadisk/PPVED-1.0 #step2: Download and decompress UbPred cd software mkdir UbPredDT cd UbPredDT wget -c http://www.ppved.org.cn/dependent/dependent_software/UbPredDTFileLinux.zip unzip UbPredDTFileLinux.zip #step3: Install UbPred wget -c http://www.ppved.org.cn/dependent/other_programs/MCR.tar.gz tar zxvf MCR.tar.gz echo "export MCRHOME=/datadisk/PPVED-1.0/software/UbPredDT/MCR/v78" >> ~/.bashrc #note: modify '/datadisk/PPVED-1.0' to your own path echo "export LD_LIBRARY_PATH=\$MCRHOME/runtime/glnx86:\$LD_LIBRARY_PATH" >> ~/.bashrc echo "export LD_LIBRARY_PATH=\$MCRHOME/bin/glnx86:\$LD_LIBRARY_PATH" >> ~/.bashrc echo "export LD_LIBRARY_PATH=\$MCRHOME/sys/os/glnx86:\$LD_LIBRARY_PATH" >> ~/.bashrc echo "export LD_LIBRARY_PATH=\$MCRHOME/sys/java/jre/glnx86/jre1.6.0/lib/i386:\$LD_LIBRARY_PATH" >> ~/.bashrc echo "export LD_LIBRARY_PATH=\$MCRHOME/sys/java/jre/glnx86/jre1.6.0/lib/i386/native_threads:\$LD_LIBRARY_PATH" >> ~/.bashrc echo "export LD_LIBRARY_PATH=\$MCRHOME/sys/java/jre/glnx86/jre1.6.0/lib/i386/server:\$LD_LIBRARY_PATH" >> ~/.bashrc echo "export XAPPLRESDIR=\$MCRHOME/X11/app-defaults" >> ~/.bashrc source ~/.bashrc chmod 755 UbPredDTFile cp ../../library/lib*.so.? . #step4: test UbPred ./UbPredDTFile s.txt test.output dos2unix p.txt #note: 'p.txt' is a file in windows format, which is converted to linux format here #results: the output file 'test.output' should be identical to the provided file 'p.txt'. Congratulations, the installation is complete !