NAME: NetPhos 3.1 FUNCTION: predict protein phosphorylation sites NOTE: assume that the path of PPVED is '/datadisk/PPVED-1.0' ================================================================ #step1: Change the current working directory to the path of PPVED cd /datadisk/PPVED-1.0 #step2: Download and decompress NetPhos cd software #you need an academic application to get the download address of NetPhos (https://services.healthtech.dtu.dk/service.php?NetPhos-3.1) e.g. : you got a download address https://services.healthtech.dtu.dk/download/6819b825-dd2e-41e9-a66b-86eb7a49f949/netphos-3.1.Linux.tar.Z wget -c https://services.healthtech.dtu.dk/download/6819b825-dd2e-41e9-a66b-86eb7a49f949/netphos-3.1.Linux.tar.Z tar zxvf netphos-3.1.Linux.tar.Z #step3: Install NetPhos cd ape-1.0 modify lines 21, 25, 28, 31 and 49 of file 'ape', and the modified contents look like this: line 21 = setenv APE /datadisk/PPVED-1.0/software/ape-1.0 line 25 = setenv ARCH i386 line 28 = setenv TMP /datadisk/PPVED-1.0/software/ape-1.0/tmp line 31 = setenv MAXCPU 8 line 49 = setenv PYTHON /usr/bin/python #step4: test NetPhos cp ../testdata/test.fa . cp ../testdata/test.netphos . ./ape test.fa >test.output #results: the output file 'test.output' should be identical to the provided file 'test.netphos'. Congratulations, the installation is complete !